Also known as: IGH/BCL2 FISH, t(14;18) FISH« Back to test list
Gene fusions involving the IGH gene at 14q32 and BCL2 at 18q21 are common in follicular lymphomas, and can also occur in diffuse large B-cell lymphomas (DLBCL). The identification of such fusions can assist in diagnosis and classification of disease.
Presence of an IGH-BCL2 gene fusion can be useful in diagnosing and sub-classifying B-NHLs. In association with BCL6 and MYC gene fusions (see ‘double/triple hit lymphoma panel’), this fusion can also indicate prognosis in B-cell lymphoma with features intermediate between DLBCL and Burkitt lymphoma.
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to identify fusion of IGH with BCL2, due to a t(14;18)(q32;q21) translocation.
This test is usually requested by a haematologist or oncologist.
0.5 mL bone marrow in transport media, or 5-10 mL blood in lithium heparin.
To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.
We also recommend that the patient or another adult check the labelling of request forms and sample tubes.