Also known as: t(12;16) FISH« Back to test list
The t(12;16) translocation results in fusion of the FUS and DDIT3 genes. This test has diagnostic implications.
FUS-DDIT3 fusion or variants having FUS rearrangement with other partners are seen in about 90% of myxoid liposarcomas, and can be useful as a diagnostic aid.
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis using probes to detect the FUS-DDIT3 fusion gene. The probes used are individual breakapart probes for FUS and DDIT3, so variants are detected.
This test is usually requested by an oncologist.
Formalin fixed, paraffin-embedded tissue (FFPE) – 10 sections of 4 uM thickness, dried overnight at 37 degrees onto charged/coated slides.