Ewing Sarcoma FISH
Also known as: Ewing's FISH, t(11;22) FISH« Back to test list
The EWSR1 probe detects breakage of this locus on chromosome 22. There are a number of possible partner genes, the most frequent being FLI1 on chromosome 11. This test has diagnostic and prognostic implications.
Fusion of EWSR1 with the FLI1 gene, or variant partners ERG, ETV1, FEV and ETV4, are all associated with Ewing sarcoma. EWSR1 can also be involved in a rearrangement with WT1, which is associated with desmoplastic small round cell tumours, ATF1 in clear cell sarcomas of soft tissue, and NR4A3 in extraskeletal myxoid chondrosarcoma.
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to detect gene fusions involving EWSR1.
This test is usually requested by an oncologist.
Formalin fixed, paraffin-embedded tissue (FFPE) – 10 sections of 4 uM thickness, dried overnight at 37 degrees onto charged/coated slides.