Also known as: BCL6 FISH, 3q27 FISH« Back to test list
Gene fusions involving the BCL6 gene at 3q27 can occur in B-cell non-Hodgkin lymphomas (B-NHL), particularly diffuse large B-cell lymphoma (DLBCL). BCL6 gene fusions are also seen in patients with follicular lymphoma or marginal zone lymphoma. These gene fusions have diagnostic and prognostic implications.
Presence of a BCL6 gene fusion can be useful in diagnosing and sub-classifying B-NHLs. It can also indicate prognosis in B-cell lymphoma with features intermediate between DLBCL and Burkitt lymphoma, in association with BCL2 and MYC gene fusions (see double/triple hit lymphoma panel).
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to identify fusion of BCL6 with a partner gene.
This test is usually requested by a haematologist or oncologist.
0.5 mL bone marrow in transport media, or 5-10 mL blood in lithium heparin.
To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.
We also recommend that the patient or another adult check the labelling of request forms and sample tubes.