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Also known as: Papillary renal cell carcinoma FISH, alveolar soft-part sarcoma FISH

Test category:

Oncology - Renal cancer

Use of test


The translocation fusing TFE3 at Xp11.2 with PRCC at 1q21.2 is seen in papillary renal cell carcinoma (PRCC). The translocation fusing TFE3 with ASPSCR1 at 17q25 is associated with alveolar soft-part sarcoma (ASPS). This test detects rearrangement of the TFE3 locus.


This test has diagnostic implications.

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.


Fluorescent in situ hybridisation (FISH) analysis of the TFE3 gene.

Requesting the test


This test is usually requested by an oncologist.

Request form:

Download the solid tumour somatic mutation request form.

Sample required:

Formalin-fixed, paraffin embedded tissue (FFPE) - 15 sections of 4 uM thickness, dried overnight at 37°C onto charged/coated slides.

Special instructions:

Please stain the first section with H&E, and label each slide with patient and block identifiers. Label the unstained sections sequentially (slides 2-15). The request should be accompanied by the somatic mutation request form (above) and a copy of the original histopathology report.

Turnaround time:

5 business days.


Up to $255.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.


This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.

Click here for our billing policy.