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SS18 FISH

Also known as: t(X;18) FISH

Test category:

Oncology - Sarcoma

Use of test

Purpose:

The t(X;18) translocation results in fusion of the SS18 gene on Chromosome 18 with one of three related genes on the short arm of the X. The probe determines breakage of SS18. This test has diagnostic implications.

Utility:

The rearrangement is specific for synovial sarcoma, and occurs in around 80% of these tumours.

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.

Methodology:

Fluorescent in situ hybridisation (FISH) analysis, using probes to detect gene fusions involving the SS18 gene.

Requesting the test

Ordering:

This test is usually requested by an oncologist.

Request form:

Download the solid tumour somatic mutation request form.

Sample required:

Formalin-fixed, paraffin embedded tissue (FFPE) - 15 sections of 4 uM thickness, dried overnight at 37°C onto charged/coated slides.

Special insructions:

Please stain the first section with H&E, and label each slide with patient and block identifiers. Label the unstained sections sequentially (slides 2-15). The request should be accompanied by the somatic mutation request form (above) and a copy of the original histopathology report.

Turnaround time:

5 business days.

Price:

Up to $410.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.

Rebate:

This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.

Click here for our billing policy.