Also known as:Hyalinising clear cell carcinoma of the salivary gland FISH, EWSR1-ATF1 FISH
Oncology - Sarcoma
Use of test
Hyalinising clear cell carcinoma of the salivary gland is characterised by a specific gene fusion, EWSR1-ATF1. The EWSR1 FISH probe detects breakage of this locus on Chromosome 22, indicating that a rearrangement has occurred.
This test has diagnostic implications. Diagnosis of salivary gland neoplasms can be complex, and present of a pathognomic gene fusion can help to determine a diagnosis. The EWSR1 FISH probe detects rearrangement involving this gene. Although the probe does not detect the specific partner gene, presence of an EWSR1 rearrangement in a salivary tumour strongly supports a diagnosis of clear cell carcinoma.
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to detect gene fusions involving EWSR1. This test is performed on thin sections of tissue, so some cells in the section may be sliced by the process, and only contain some of the regions of interest. Sections may contain bony, fatty or necrotic material, which may interfere with processing and/or analysis. Dense tissue also makes identification of individual cells difficult. The accuracy of the results is dependent on the correct identification of tumour on the sections provided, and the assumption that positive results will be identified by a large proportion of cells within the tumour having a rearrangement.
Formalin-fixed, paraffin embedded tissue (FFPE) - 15 sections of 4 uM thickness, dried overnight at 37°C onto charged/coated slides.
Please stain the first section with H&E, and label each slide with patient and block identifiers. Label the unstained sections sequentially (slides 2-15). The request should be accompanied by the somatic mutation request form (above) and a copy of the original histopathology report.
Up to 4 weeks.
Up to $340.
If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.
An MBS rebate is available if criteria are met (item number 73382).