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PTEN FISH

Also known as: PTEN deletion FISH

Test category:

Oncology - Brain cancer

Use of test

Purpose:

The Phosphate and Tensin homologue (PTEN) gene on 10q23 is mutated in a wide range of cancers. Allelic loss is a frequent event in glioblastomas. This test has diagnostic implications.

Utility:

PTEN deletion occurs in around 30% of glioblastomas. Establishing the presence of the deletion can be an aid to diagnosis.

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.

Methodology:

Fluorescent in situ hybridisation (FISH) analysis, using probes to enumerate the PTEN gene.

Requesting the test

Ordering:

This test is usually requested by an oncologist.

Request form:

>Solid Tumour somatic mutation request form

Sample required:

Formalin-fixed, paraffin embedded tissue (FFPE) - 15 sections of 4 uM thickness, dried overnight at 37°C onto charged/coated slides.

Special instructions:

Please stain the first section with H&E, and label each slide with patient and block identifiers. Label the unstained sections sequentially (slides 2-15). The request should be accompanied by the somatic mutation request form (above) and a copy of the original histopathology report.

Turnaround time:

5 business days.

Price:

Up to $255.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.

Rebate:

This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.

Click here for our billing policy.