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Also known as: PTEN deletion FISH

Test category:

Oncology - Brain cancer

Use of test


The Phosphate and Tensin homologue (PTEN) gene on 10q23 is mutated in a wide range of cancers. Allelic loss is a frequent event in glioblastomas. This test has diagnostic implications.


PTEN deletion occurs in around 30% of glioblastomas. Establishing the presence of the deletion can be an aid to diagnosis.

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.


Fluorescent in situ hybridisation (FISH) analysis, using probes to enumerate the PTEN gene.

Requesting the test


This test is usually requested by an oncologist.

Request form:

>Solid Tumour somatic mutation request form

Sample required:

Formalin-fixed, paraffin embedded tissue (FFPE) - 15 sections of 4 uM thickness, dried overnight at 37°C onto charged/coated slides.

Special instructions:

Please stain the first section with H&E, and label each slide with patient and block identifiers. Label the unstained sections sequentially (slides 2-15). The request should be accompanied by the somatic mutation request form (above) and a copy of the original histopathology report.

Turnaround time:

5 business days.


Up to $255.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.


This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.

Click here for our billing policy.