Back to test list

IGH/BCL2 FISH

Also known as: t(14;18) FISH

Test category:

Oncology - Lymphoma

Use of test

Purpose:

Gene fusions involving the IGH gene at 14q32 and BCL2 at 18q21 are common in follicular lymphomas, and can also occur in diffuse large B-cell lymphomas (DLBCL). The identification of such fusions can assist in diagnosis and classification of disease.

Utility:

Presence of an IGH-BCL2 gene fusion can be useful in diagnosing and sub-classifying B-NHLs. In association with BCL6 and MYC gene fusions (see Multiple hit lymphoma FISH), this fusion can also indicate prognosis in B-cell lymphoma with features intermediate between DLBCL and Burkitt lymphoma.

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.

Methodology:

Fluorescent in situ hybridisation (FISH) analysis, using probes to identify fusion of IGH with BCL2, due to a t(14;18)(q32;q21) translocation.

Requesting the Test

Ordering:

This test is usually requested by a haematologist or oncologist.

Sample required:

0.5 mL bone marrow in transport media, or formalin-fixed, paraffin embedded tissue (FFPE).

To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.

We recommend that the patient or another adult check the labelling of request forms and sample tubes.

Turnaround time:

2 business days.

Price:

Up to $255.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.

Rebate:

This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.