Also known as:t(8;14) FISH, IGH-MYC FISH, MYC-IGH FISH
Oncology - Lymphoma
Use of test
Gene fusions involving the IGH gene at 14q32 and MYC at 8q24 are common in Burkitt lymphoma. They can also occur in other B-cell non-Hodgkin lymphomas, for example, diffuse large B-cell lymphoma (DLBCL). Less commonly, MYC can be fused with other gene partners, such as IGK and IGL. This testing is also available.
Such gene fusions have diagnostic and prognostic implications.
Presence of an IGH-MYC gene fusion can be useful in diagnosing Burkitt lymphoma. In association with BCL2 and BLC6 gene fusions (see Multiple hit lymphoma FISH), this fusion can also indicate prognosis in B-cell lymphoma with features intermediate between DLBCL and Burkitt lymphoma.
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to identify fusion of IGH with MYC, due to a t(8;14)(q24;q32) translocation. If performed on FFPE tissue, some cells in the section may be sliced by the process, and only contain some of the regions of interest. Sections may contain bony, fatty or necrotic material, which may interfere with processing and/or analysis. Dense tissue also makes identification of individual cells difficult. The accuracy of the results is dependent on the correct identification of tumour on the sections provided, and the assumption that positive results will be identified by a large proportion of cells within the tumour having a rearrangement.
Requesting the test
This test is usually requested by a haematologist or oncologist.
0.5 mL bone marrow in transport media, or formalin-fixed, paraffin embedded tissue (FFPE).
To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.
We recommend that the patient or another adult check the labelling of request forms and sample tubes.
If requesting on FFPE, please label each slide with patient and block identifiers plus number them sequentially 1-15. Stain slides 1 and 15 with H&E and leave slides 2-14 unstained.
Up to 4 weeks.
If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.
An MBS rebate is available for Burkitt lymphoma (item number 73365), or diffuse large B-cell lymphoma/high-grade B-cell lymphomas as part of the Multiple hit lymphoma FISH panel (item number 73364), if criteria are met.