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BCL6 FISH

Also known as: 3q27 FISH, B-cell non-Hodgkin lymphomas

Test category:

Oncology - Lymphoma

Use of test

Purpose:

Gene fusions involving the BCL6 gene at 3q27 can occur in B-cell non-Hodgkin lymphomas (B-NHL), particularly diffuse large B-cell lymphoma (DLBCL). BCL6 gene fusions are also seen in patients with follicular lymphoma or marginal zone lymphoma. These gene fusions have diagnostic and prognostic implications.

Utility:

Presence of a BCL6 gene fusion can be useful in diagnosing and sub-classifying B-NHLs. It can also indicate prognosis in B-cell lymphoma with features intermediate between DLBCL and Burkitt lymphoma, in association with BCL2 and MYC gene fusions (see Multiple hit lymphoma FISH).

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.

Methodology:

Fluorescent in situ hybridisation (FISH) analysis, using probes to identify fusion of BCL6 with a partner gene. The breakpoints involved in these fusions can be variable, and in some cases certain variants will not be detected by the probe.

Requesting the test

Ordering:

This test is usually requested by a haematologist or oncologist.

Sample required:

0.5 mL bone marrow in transport media, or formalin-fixed, paraffin embedded tissue (FFPE).

To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.

We recommend that the patient or another adult check the labelling of request forms and sample tubes.

Turnaround time:

5 business days.

Price:

Up to $255.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.

Rebate:

This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.