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6q21 deletion FISH

Test category:

Oncology - Lymphoma

Use of test

Purpose:

Deletions affecting the long arm of Chromosome 6, including the 6q21 region, are common in mature B-cell lymphoid neoplasms, for example, plasma cell neoplasms, mantle cell lymphoma, and B-cell non-Hodgkin lymphoma, particularly lymphoplasmacytic lymphoma. These deletions can assist in subsequent monitoring of disease.

Utility:

Presence of a 6q21 deletion is not prognostically significant, but can be a marker for presence of a monoclonal population, or for quantitative population size estimation.

Ethical considerations:

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.

Methodology:

Fluorescent in situ hybridisation (FISH) analysis, using probes located at 6q21. This interphase FISH test has a cut off at 5%. A low level of positive cells may be due to overlap of the two signals. Studies of normal controls indicate that this level is less than 5%. Small abnormal populations may therefore not be detectable.

Requesting the test

Ordering:

This test is usually requested by a haematologist or oncologist.

Sample required:

0.5 mL bone marrow in transport media, or 5-10 mL blood in lithium heparin.

To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.

We recommend that the patient or another adult check the labelling of request forms and sample tubes.

Turnaround time:

5 business days.

Price:

Up to $255.

If more information is required, the requesting clinician can call our laboratory on (07) 3377 8573.

Rebate:

This test is not rebated by Medicare. The laboratory assumes that the patient or client has provided informed financial consent for the test.