Multiple Hit Lymphoma FISH
Also known as: Double/Triple Hit Lymphoma Panel« Back to test list
A B-cell lymphoma may exhibit multiple gene rearrangements involving the genes MYC and BCL2 or MYC and BCL6 (double hit), or MYC and BCL2 and BCL6 (triple hit). These rearrangements have prognostic implications.
Double and triple hit lymphomas have a poor prognosis.
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to detect gene fusions/rearrangements involving the MYC, BCL2 and BCL6 genes.
This test is usually requested by a haematologist or oncologist.
0.5 mL bone marrow in transport media or 10 mL blood in lithium heparin if clinically appropriate. Testing can also be performed on formalin fixed, paraffin-embedded (FFPE) lymph node tissue.
To help ensure the quality of the test, a genetic test should be done with a dedicated sample whenever possible i.e. a sample collected specifically for that test rather than a sample that is used for multiple tests.
We also recommend that the patient or another adult check the labelling of request forms and sample tubes.