Also known as: EGFR Amplification FISH« Back to test list
Amplification of the EGFR gene, leading to overexpression, can occur in a number of tumour types. This test has diagnostic and prognostic implications in patients with glial tumours.
EGFR amplification is currently most clinically useful in brain malignancies of glial origin where it can assist in classification of the tumour. EGFR amplification is a marker of more aggressive disease, but there is conflicting evidence regarding the utility of this association.
Although EGFR amplification can occur in lung tumours, it does not currently have clinical utility in these tumours, as opposed to EGFR mutation testing (see EGFR Screen).
This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.
Fluorescent in situ hybridisation (FISH) analysis, using probes to enumerate the EGFR gene.
This test is usually requested by an oncologist.
Formalin fixed, paraffin-embedded tissue (FFPE) – 10 sections of 4 uM thickness, dried overnight at 37 degrees onto charged/coated slides.