Our Tests

BRAF Screen

Also known as: BRAF Gene Test

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Purpose

Certain mutations in the BRAF gene in some solid tumours are associated with responses to therapy or the likelihood of familial disease.

Clinical Utility

In metastatic melanoma, the presence of these mutations is associated with increased sensitivity to BRAF-inhibitor therapy. The absence of such mutations indicates that the patient’s tumour is less likely to respond.

In colorectal cancer, the absence of a BRAF mutation is associated with an increased probability of the patient having Lynch syndrome, an inherited DNA mismatch-repair deficiency.

This is an assay for non-heritable mutations. It does not raise issues of ethics or consent that are different from most other investigations ordered in the routine care of a patient.

Genes

BRAF

Test Method

Pyrosequencing analysis of codon 600 of the BRAF gene.

Test Ordering

This test is usually requested by an oncologist, surgeon or clinical geneticist. If ordered by a pathologist, the Medicare rebate may still apply. Please use the specific request form (see link below).

Formalin fixed, paraffin-embedded tissue (FFPE) – 10 sections of 4 uM thickness, dried overnight at 37 degrees onto charged/coated slides.

Special Instructions

Please stain the first section with H&E, and label each slide with patient and block identifiers. Label the unstained sections sequentially (slides 2D10). The request should be accompanied by completed Somatic Mutation Test Request Form and a copy of the original histopathology report.

Price

$230.95

This test may have a rebate for all or part of the cost.

Medicare Rebate

This test has a Medicare rebate of $196.35, subject to the requirements of the Medicare descriptor being met. The MBS item number is 73336.

Turnaround Time

10 working days.